Additional file 9: Figure S9. of Anterograde monosynaptic transneuronal tracers derived from herpes simplex virus 1 strain H129

Quantitative analysis of H129-ΔTK-tdT monosynaptic tracing. Helper AAVs and H129-ΔTK-tdT were sequentially injected at VPM (a), nRT (b) or VTA (c) of wild type C57BL/6, PV-Cre or DAT-Cre mice respectively, as described in Fig.4-6. The brains’ corona slices were observed with an interval of 120 μM (every 4 slices), and the amounts of the labeled cell at the indicated brain regions were counted. The starter cells at the injection site were detected at Day32 as tdTomato and GFP co-expressing neurons (opened circle, each circle represents one mouse), and the tdTomato positive neurons at other brain regions were observed at Day 32 (filled triangle, each circle represents one mouse). (PDF 109 kb)