Analytik von N-nitroso-verbindungen

Analysis of N-nitroso compounds. II. Separation and quantitative determination of N-nitroso-N-alkylureas and N-nitroso-N-alkylurethans by high-speed high-pressure liquid chromatography . Physical data, elementary analysis data and IR, UV, NMR and mass spectra have been determined for N-nitroso-N-alkylureas and N-nitroso-N-alkylurethans. The fragmentation patterns in the mass spectrometer induced by electron impact are discussed. A fast separation is described of model mixtures of alkyl homologous N-nitroso-N-alkylureas and N-nitroso-N-alkylurethans by reversed-phase liquid—liquid distribution in a liquid chromatograph. Detection is carried out by UV absorption at 254 nm. Calibration curves for the quantitative determination of separated N-nitroso-N-alkylureas are given. The lowest detection limit for N-nitroso-N-methylurea was 10 ng.