Fermented extract of mealworm (Tenebrio molitor larvae) as a dietary protein source modulates hepatic proteomic profiles in C57BLKS/J-db/db mice

This study hypothesised that fermented mealworm extract (TMP) with higher amino acid contents than nonfermented extract can be used as a dietary protein source for type 2 diabetes mellitus, and compared its effects with casein (CA) using C57BLKS/J-db/db mice. TMP marginally lowered serum insulin and leptin levels and hepatic triglyceride, and lipid droplets compared with CA group. Proteomic analysis showed that 14 proteins (H4C1, CYB5A, ANXA5, RGN, 40S ribosomal protein, Atp2, PDI, GRP78, RDX, GCH1, LDHA, GNMT, ACAA2, and UROC1) were up-regulated and 8 proteins (UQCRC1, Txndc5, SBP2, HSPA8, PHB, TF, PC, and PK) were down-regulated in the CA group compared to the normal mice. However, TMP significantly down-regulated ANXA5, RGN, Atp2, PDI, RDX, LDHA, ACAA2, and UROC1, and up-regulated UQCRC1, PHB, TF, and PK. These proteins are related to glycolysis, lactate production, fatty acid β-oxidation, oxidative stress, and endoplasmic reticulum stress. These results suggest that TMP is a potential protein source that can replace CA in type 2 diabetes.