Back to Search
Start Over
O2-Dependent Protein Internalization Underlies Astrocytic Sensing of Acute Hypoxia by Restricting Multimodal TRPA1 Channel Responses
- Source :
- Current Biology. 30:3378-3396.e7
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Summary Hypoxia sensors are essential for regulating local oxygen (O2) homeostasis within the body. This is especially pertinent within the CNS, which is particularly vulnerable to O2 deprivation due to high energetic demand. Here, we reveal hypoxia-monitoring function exerted by astrocytes through an O2-regulated protein trafficking mechanism within the CNS. Strikingly, cultured mouse astrocytes isolated from the parafacial respiratory group (pFRG) and retrotrapezoid nucleus (RTN) region are capable of rapidly responding to moderate hypoxia via the sensor cation channel transient receptor potential (TRP) A1 but, unlike multimodal sensory neurons, are inert to hyperoxia and other TRPA1 activators (carbon dioxide, electrophiles, and oxidants) in normoxia. Mechanistically, O2 suppresses TRPA1 channel activity by protein internalization via O2-dependent proline hydroxylation and subsequent ubiquitination by an E3 ubiquitin ligase, NEDD4-1 (neural precursor cell-expressed developmentally down-regulated protein 4). Hypoxia inhibits this process and instantly accumulates TRPA1 proteins at the plasma membrane, inducing TRPA1-mediated Ca2+ influx that triggers ATP release from pFRG/RTN astrocytes, potentiating respiratory center activity. Furthermore, astrocyte-specific Trpa1 disruption in a mouse brainstem-spinal cord preparation impedes the amplitude augmentation of the central autonomic respiratory output during hypoxia. Thus, reversible coupling of the TRPA1 channels with O2-dependent protein translocation allows astrocytes to act as acute hypoxia sensors in the medullary respiratory center.
- Subjects :
- 0301 basic medicine
Hyperoxia
biology
media_common.quotation_subject
Respiratory center
Hypoxia (medical)
General Biochemistry, Genetics and Molecular Biology
Ubiquitin ligase
Cell biology
03 medical and health sciences
Transient receptor potential channel
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Ubiquitin
medicine
biology.protein
medicine.symptom
General Agricultural and Biological Sciences
Internalization
030217 neurology & neurosurgery
media_common
Astrocyte
Subjects
Details
- ISSN :
- 09609822
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Current Biology
- Accession number :
- edsair.doi...........bec9ebdeaff53cb7586a61d83e1bbbf7