Sex-Specific Differences In Disease Severity In Patients With Chronic Rhinosinusitis With Nasal Polyps

S U N D A Y 583 Regulation Of Expression Of Pendrin Protein In CRS With Nasal Polyps and In Airway Epithelial Cells Dr. Sudarshan Seshadri, PhD, Dr. Xiang Lu, MD, PhD, Mr. Matthew Purkey, BS, Dr. Tetsuya Homma, MD, Mr. Andrew Choi, Mr. Roderick Carter, BSc, Mr. James Norton, MS, Ms. Lydia Suh, BSc, Dr. Atsushi Kato, PhD, Prof. Pedro C. Avila, MD, FAAAAI, Dr. Anju T. Peters, MD, FAAAAI, Dr. David Conley, MD, Dr. Rakesh Chandra, MD, Dr. Bruce K. Tan, MD, Dr. Leslie C. Grammer, MD, FAAAAI, Dr. Robert C. Kern, MD, Dr. Robert P. Schleimer, PhD, FAAAAI; Department of Medicine, Division of Allergy-Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL, Department of Otolaryngology, Northwestern University Feinberg School of Medicine, Chicago, IL, Northwestern University, Chicago, IL, Division of Allergy-Immunology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL. RATIONALE: Pendrin is an anion transporter expressed by airway epithelial cells. Pendrin functions as a regulator of inflammation, mucus production and in the maintenance of air-surface liquid (ASL). We and others reported that expression of pendrin mRNA was elevated in nasal polyps (NP) of patients with chronic rhinosinusitis (CRS). In this study we analyzed the regulation of pendrin protein expression in NPs of patients with CRS and also in nasal epithelial cells (NECs). METHODS: Tissue samples were collected and analyzed by real-time PCR and/or immunoblot analysis for expression of pendrin and various cytokines. NECs were stimulated with various cytokines, dsRNA and rhinovirus to analyze the regulation of pendrin expression. RESULTS: Immunoblot analysis indicated that pendrin protein was increased in NP of patients with CRS compared to control uncinates (3.5 fold, p