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Transformation of wheat Triticum aestivum with the HvBADH1 transgene from hulless barley improves salinity-stress tolerance
- Source :
- Acta Physiologiae Plantarum. 41
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Studies have shown that the stress tolerance of cereal plants to osmotic or salinity stresses can be improved to varying degrees by the overexpression of an introduced betaine aldehyde dehydrogenase (BADH) gene. In the present study, the HvBADH1 gene from Hordeum vulgare L. var. nudum Hook. f., encoding a cytosolic BADH, was transferred into Triticum aestivum via traditional Agrobacterium tumefaciens-mediated transformation. Molecular methods, such as PCR, Southern blot analysis, and real-time quantitative RT-PCR were used to identify the successful integration and expression of the HvBADH1 transgene in genetically transformed wheat lines. To detect the efficacy of the HvBADH1 transgene in the transformants, some pivotal physiological indicators that reflected abiotic stress tolerance were measured in individual transgenic plant lines. These indicators included intracellular K+ and Na+ contents or K+/Na+ ratio, relative conductivity, and malondialdehyde and glycine betaine (GB) concentrations in cells. The results revealed that all the tested transgenic lines could significantly increase the recruitments of K+ in their cytosol than the wild-type seedlings. Similarly, 11.59- to 21.82-fold greater accumulation of GB, 2.11–2.56 times higher calli relative growth rates, and 26.2–29.1% seedling survival rates were found in transgenic lines under 150 mM NaCl stressed conditions. Our results demonstrated that by overexpressing the HvBADH1 transgene in genetically transformed wheat, the overall salt tolerance of the target plants was significantly increased, and the damaging effects of high salinity were significantly reduced.
- Subjects :
- 0106 biological sciences
0301 basic medicine
biology
Physiology
Agrobacterium
Abiotic stress
Transgene
food and beverages
Plant Science
biology.organism_classification
01 natural sciences
Salinity
03 medical and health sciences
Horticulture
Transformation (genetics)
chemistry.chemical_compound
030104 developmental biology
Betaine
chemistry
Betaine-aldehyde dehydrogenase
Hordeum vulgare
Agronomy and Crop Science
010606 plant biology & botany
Subjects
Details
- ISSN :
- 18611664 and 01375881
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Acta Physiologiae Plantarum
- Accession number :
- edsair.doi...........bf59da9eec4b36cd073d0de9108704a8
- Full Text :
- https://doi.org/10.1007/s11738-019-2940-8