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Computational single-objective scanning light sheet (cSOLS)

Authors :
Tienan Xu
Hanqi Lin
Yean J. Lim
Philip R. Nicovich
Katharina Gaus
Woei Ming Lee
Source :
APL Photonics. 7:081302
Publication Year :
2022
Publisher :
AIP Publishing, 2022.

Abstract

Single-objective scanning light sheet (SOLS) imaging has fueled major advances in volumetric bioimaging because it supports low phototoxic, high-resolution imaging over an extended period. The remote imaging unit in the SOLS does not use a conventional epifluorescence image detection scheme (a single tube lens). In this paper, we propose a technique called the computational SOLS (cSOLS) that achieves light sheet imaging without the remote imaging unit. Using a single microlens array after the tube lens (lightfield imaging), the cSOLS is immediately compatible with conventional epifluorescence detection. The core of cSOLS is a Fast Optical Ray (FOR) model. FOR generates 3D imaging volume (40 × 40 × 14 µm3) using 2D lightfield images taken under SOLS illumination within 0.5 s on a standard central processing unit (CPU) without multicore parallel processing. In comparison with traditional lightfield retrieval approaches, FOR reassigns fluorescence photons and removes out-of-focus light to improve optical sectioning by a factor of 2, thereby achieving a spatial resolution of 1.59 × 1.92 × 1.39 µm3. cSOLS with FOR can be tuned over a range of oblique illumination angles and directions and, therefore, paves the way for next-generation SOLS imaging. cSOLS marks an important and exciting development of SOLS imaging with computational imaging capabilities.

Details

ISSN :
23780967
Volume :
7
Database :
OpenAIRE
Journal :
APL Photonics
Accession number :
edsair.doi...........bfee2b3854014f493a4271b7cc8627cd