Point-of-Care Testing System of Uric Acid for the Prevention from Urolithiasis Recurrence

In this study, a simple, novel and inexpensive third generation electrochemical uric acid (UA) biosensor based on three-electrode point-of-care test strip was proposed. On the low-cost screen-printed three silver-based electrodes test strip, the Ag/AgCl reference electrode was first formed by simple electrodeposition with one silver electrode, then the working electrode was prepared by the successive coating of redox mediator, polymer hydrogel, and uricase onto the surface of another silver electrode, the third bare silver electrode was served as the counter electrode to complete the uric acid test strip, which has the advantages of lower oxidation potential, faster response time, higher sensitivity, and wider detecting range.