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Seeding Protein Crystallization with Cross-Linked Protein Crystals
- Source :
- Crystal Growth & Design. 18:1090-1100
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Protein crystallization is of great importance because protein crystals have a number of different important applications, including large-scale purification of proteins, determination of protein structure, nanoparticle preparation, and theoretical studies of crystallization. An approach often used to efficiently crystallize proteins is the use of nucleants or seeds (small fragments of protein crystals) that can help increase the probability of protein crystallization. Due to the very positive effect that seeding has on protein crystallization, seeds are now widely accepted and utilized in practical protein crystallization. Here, we show that cross-linked protein crystals (CLPCs), which retain the crystal structure but are much more stable than non-cross-linked crystals, can also be used as a new type of seed for promoting protein crystallization. Seeding with CLPCs has effects on both the reproducibility and screening of protein crystals and could improve the optical perfection (well-defined facets) of p...
- Subjects :
- 0301 basic medicine
Materials science
Linked protein
food and beverages
Nanoparticle
02 engineering and technology
General Chemistry
Crystal structure
021001 nanoscience & nanotechnology
Condensed Matter Physics
law.invention
03 medical and health sciences
030104 developmental biology
Protein structure
Chemical engineering
law
General Materials Science
Seeding
Crystallization
0210 nano-technology
Protein crystallization
Subjects
Details
- ISSN :
- 15287505 and 15287483
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Crystal Growth & Design
- Accession number :
- edsair.doi...........c03a3331c98a1dafda6d024d6ad4bbf7