Image Visualization and Proteoform Assignment of MALDI-MSI from LCMS Experimental Databases v1

Scope: The protocol describes a workflow for processing mass spectrometry imaging (MSI) data of high resolution intact proteins/proteoforms by MALDI. It involves the visualization and image generation using commercial software and also peak annotation using open source code. Expected Outcomes: Tabular output of matched proteoforms, as well as a trelliscope display of overlaid isotopic distributions allowing for the annotation of high-resolution accurate mass distributions of proteoforms.