SRY and DelE Detection in Polled Intersex Tangshan Dairy Goat

The sex determining region Y (SRY) gene and deletion element DelE were analyzed in 34 polled intersex syndrome (PIS) or 6 normal Tangshan dairy goats (3 females and 3 males) by PCR technology. The result showed that the special male SRY gene only existed in male goats, and was absent in all PIS goats or normal female goats. The 28 PIS goats were homozygote for DelE, and the other 6 goats were heterozygote for DelE deletion or homozygote for non DelE deletion. It could be concluded that the PIS Tangshan dairy goats were female for its genetic basis and the DelE was partial deletion.