Thiotipin, a novel thiopeptide with a tipA promoter inducing activity produced by Streptomyces sp. DT31

Thiotipin was isolated from the mycelium of Streptomyces sp. DT31 as a tipA promoter inducing substance. Based on various NMR studies including 1 H- 1 H COSY, HSQC, FG-HMBC, phase-sensitive 13 C-decoupled HMBC (D-HMBC) and NOE, its structure was established as a thiopeptide with oxazoles, thiazoles and several modified amino acids. Minimum induction concentration of thiotipin for tipA promoter was 80 ng/ml.