Additional file 9: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Figure S6 Differentiated U937 cells transfected with an empty vector (EV) or a granulin (GRN) expression vector and subsequently cultivated in the presence of CM from MDA-MB-468 cells were evaluated for their differentiation state (percentage of CD11b+ cells) (a) and for their viability (b) by, respectively, FACS analysis and ATPlite assay at the indicated time points after CM addition. c Overexpression of GRN evaluated by Western blotting. (PDF 141 kb)