Gamma radiation on silver nano colloid for anti-fungal activity

Soft gamma ray irradiation is done on Silver nano colloids prepared using Pepper Nigrum as reducing and stabilizing agent. Change in surface morphology and the structure of the colloid is obtained from Transmission Electron Microscope images and Selected area Electron Diffractogram. Morphology of the gamma irradiated sample is devoid of nano particle clusters and the Selected Area Electron Diffraction pattern confirms the crystal nature as face centered cubic structure of nano silver. UV–visible spectroscopic analysis gives the absorption peaks in the green region. Gamma irradiation results in the reduction in peak intensity without any change in wavelength. A statistical approach is done on treating the gamma irradiated colloidal sample with the as prepared silver nano colloid for measuring the area of affecting fungal infection on a potato-agar medium. It is found that the fungal growth is declined rapidly with the introduction of gamma irradiated silver nano colloid than the as prepared colloid.