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Kinetic studies on poly-3-hydroxybutyrate formation by Ralstonia eutropha in a two-stage continuous culture system
- Source :
- Process Biochemistry. 37:219-227
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- A continuous culture system consisting of two chemostats in series was used to investigate the kinetics of poly-3-hydroxybutyrate (PHB) formation by Ralstonia eutropha . The cells of R. eutropha grew under glucose-limited conditions in the first-stage and accumulated PHB under nitrogen-limited conditions in the second-stage. The carbon/nitrogen ( C / N ) ratio in the second fermenter was controlled by feeding the fermenter with a fresh glucose solution. The biokinetics of R. eutropha in different nutrient conditions were studied at various steady states by controlling the dilution rates. The cell growth, glucose utilization and PHB formation under the nitrogen-rich conditions followed a Monod model very well. PHB was accumulated substantially under nitrogen-limited conditions, and the specific PHB synthetic rate was dependent on C / N molar ratio, which also closely approximated a Monod model.
- Subjects :
- Glucose utilization
Chromatography
biology
Chemistry
Kinetics
technology, industry, and agriculture
Poly-3-hydroxybutyrate
chemistry.chemical_element
Bioengineering
Industrial fermentation
macromolecular substances
equipment and supplies
biology.organism_classification
Applied Microbiology and Biotechnology
Biochemistry
Nitrogen
Dilution
Ralstonia
lipids (amino acids, peptides, and proteins)
Eutropha
Subjects
Details
- ISSN :
- 13595113
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Process Biochemistry
- Accession number :
- edsair.doi...........c4c236d05f3c6301c4b99b40b6cc2a9e