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Immunocytochemical characterisation of neural stem-progenitor cells from green terror cichlidAequidens rivulatus

Authors :
C M Wen
M. M. Chen
Fan-Hua Nan
C. S. Wang
Source :
Journal of Fish Biology. 90:201-221
Publication Year :
2016
Publisher :
Wiley, 2016.

Abstract

In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein 32 (DARPP-32), oligodendrocyte transcription factor 2, paired box 6 and sex determining region Y-box 2. The intensity and localisation of these proteins, however, varied among the different NSPCs. Despite being intermediate cells, NSPCs can be divided into radial glial cells, oligodendrocyte progenitor cells (OPC) and neuroblasts by expressing the astrocyte marker glial fibrillary acidic protein (GFAP), OPC marker A2B5 and neuronal markers, including acetyl-tubulin, βIII-tubulin, microtubule-associated protein 2 and neurofilament protein. Nevertheless, astrocytes were polymorphic and were the most dominant cells in the NSPC cultures. By using Matrigel, radial glia exhibiting a long GFAP+ or DARPP-32+ fibre and neurons exhibiting a significant acetyl-tubulin+ process were obtained. The results confirmed that NSPCs obtained from A. rivulatus brains can proliferate and differentiate into neurons in vitro. Clonal culture can be useful for further studying the distinct NSPCs.

Details

ISSN :
00221112
Volume :
90
Database :
OpenAIRE
Journal :
Journal of Fish Biology
Accession number :
edsair.doi...........c577106a236b99b4e9414ffbcf5af124
Full Text :
https://doi.org/10.1111/jfb.13170