Evidence for two different P2X -receptors mediating vasoconstriction of Ap5 A and Ap6 A in the isolated perfused rat kidney

The activation of various P2-receptor subtypes in rat renal vasculature by P1, P5-diadenosine pentaphosphate (Ap5A) and P1, P6-diadenosine hexaphosphate (Ap6A) were studied by measuring their effects on perfusion pressure during continuous perfusion in a rat isolated perfused kidney. Permanent perfusion with Ap5A and Ap6A elicited both a transient and sustained vasoconstriction with both vasoconstrictions to be different: the transient vasoconstriction can be elicited with concentrations 10 nM, whereas the sustained vasoconstriction is observed with concentrations 1 nM. Ap5A and Ap6A act via the same receptors as α,β-methylene ATP (α,β-meATP). The rank order of potency for transient vasconstriction was α,β-meATP=Ap5A>Ap6A>β,γ-meATP, and for sustained vasoconstriction α,β-meATP=Ap5A>β,γ-meATP Ap6A. Suramin, a non-selective P2-receptor antagonist, and pyridoxal-phosphate-6-azophenyl-2;4-disulphonic acid (PPADS) a highly selective P2X-receptor antagonist antagonized both the transient and the sustained vasoconstriction. Taken together the results of the agonist profile of Ap5A and Ap6A and comparing its findings to literature it can be demonstrated that the transient but not the sustained vasoconstriction is mediated via the P2X1-receptor which is present in rat renal vasculature. It is demonstrated that the agonist profile of the sustained vasoconstriction induced by Ap5A and Ap6A does not fit to any currently known P2X- or P2Y-receptor subtype. We conclude a yet unidentified P2X-receptor or chimeric P2X-receptor may contribute to the effects on rat renal vasculature produced by Ap5A and Ap6A and which may play an important role in glomerular perfusion pressure and blood pressure control. British Journal of Pharmacology (1999) 127, 1463–1469; doi:10.1038/sj.bjp.0702667