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Ruthenium-caged antisense morpholinos for regulating gene expression in zebrafish embryos
- Source :
- Chemical Science. 6:2342-2346
- Publication Year :
- 2015
- Publisher :
- Royal Society of Chemistry (RSC), 2015.
-
Abstract
- Photochemical approaches afford high spatiotemporal control over molecular structure and function, for broad applications in materials and biological science. Here, we present the first example of a visible light responsive ruthenium-based photolinker, Ru(bipyridine)2(3-ethynylpyridine)2 (RuBEP), which was reacted stoichiometrically with a 25mer DNA or morpholino (MO) oligonucleotide functionalized with 3′ and 5′ terminal azides, via Cu(I)-mediated [3+2] Huisgen cycloaddition reactions. RuBEP-caged circular morpholinos (Ru-MOs) targeting two early developmental zebrafish genes, chordin and notail, were synthesized and tested in vivo. One-cell-stage zebrafish embryos microinjected with Ru-MO and incubated in the dark for 24 h developed normally, consistent with caging, whereas irradiation at 450 nm dissociated one 3-ethynylpyridine ligand (Φ = 0.33) and uncaged the MO to achieve gene knockdown. As demonstrated, Ru photolinkers provide a versatile method for controlling structure and function of biopolymers.
- Subjects :
- Genetics
Gene knockdown
animal structures
biology
Morpholino
010405 organic chemistry
Oligonucleotide
chemistry.chemical_element
General Chemistry
010402 general chemistry
biology.organism_classification
Ligand (biochemistry)
01 natural sciences
0104 chemical sciences
Ruthenium
Bipyridine
chemistry.chemical_compound
chemistry
embryonic structures
Biophysics
Chordin
Zebrafish
Subjects
Details
- ISSN :
- 20416539 and 20416520
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Chemical Science
- Accession number :
- edsair.doi...........c6a904ebd4cec0033c22bdf95049fa5c