Study on the Extraction of Components from H.littoralis

The purpose of this study is to investigate the extraction and separation of alkaloids from H.littoralis. Chopped bulbs of H.littoralis was soaked in a mixture of dichloromethane and methanol (1:1) for 24 h, ultrasonic extraction six times, then extracted with N-butanol to yield crude extracts. Three components were isolated from N-butanol extract of H.littoralis by Sephadex LH-20 and TLC. The compounds were identified by LC-MS, they are 5,6-dihydrobicolorine, Lycorine and 7-deoxy-trans-dihydronarciclasine.