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Genome-wide identification and expression profiling of chitinase genes in tea (Camellia sinensis (L.) O. Kuntze) under biotic stress conditions
- Source :
- Physiology and Molecular Biology of Plants. 27:369-385
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Chitinases are a diverse group of enzymes having the ability to degrade chitin. Chitin is the second most abundant polysaccharide on earth, predominantly found in insect exoskeletons and fungal cell walls. In this study, we performed a genome-wide search for chitinase genes and identified a total of 49 chitinases in tea. These genes were categorized into 5 classes, where an expansion of class V chitinases has been observed in comparison to other plant species. Extensive loss of introns in 46% of the GH18 chitinases indicates that an evolutionary pressure is acting upon these genes to lose introns for rapid gene expression. The promoter upstream regions in 65% of the predicted chitinases contain methyl-jasmonate, salicylic acid and defense responsive cis-acting elements, which may further illustrate the possible role of chitinases in tea plant's defense against various pests and pathogens. Differential expression analysis revealed that transcripts of two GH19 chitinases TEA028279 and TEA019397 got upregulated during three different fungal infections in tea. While GH19 chitinase TEA031377 showed an increase in transcript abundance in the two insect infested tea tissues. Semi-quantitative RT-PCR analysis revealed that five GH19 chitinases viz. TEA018892, TEA031484, TEA28279, TEA033470 and TEA031277 showed significant increase in expression in the tea plants challenged with a biotrophic pathogen Exobasidium vexans. The study endeavours in highlighting biotic stress responsive defensive role of chitinase genes in tea.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Physiology
Plant Science
Biology
Polysaccharide
01 natural sciences
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Exobasidium vexans
Chitin
Gene expression
Camellia sinensis
Molecular Biology
Gene
chemistry.chemical_classification
fungi
food and beverages
Biotic stress
biology.organism_classification
030104 developmental biology
chemistry
Chitinase
biology.protein
010606 plant biology & botany
Subjects
Details
- ISSN :
- 09740430 and 09715894
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Physiology and Molecular Biology of Plants
- Accession number :
- edsair.doi...........c8004d5094fa2c499878e231d31f4930