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Hybridization of Euglena RNA to the heavy and the light chloroplast DNA components separated in alkaline CsC1 gradients

Authors :
James R. Y. Rawson
Cindy L. Boerma
Source :
Biochemical and Biophysical Research Communications. 74:912-918
Publication Year :
1977
Publisher :
Elsevier BV, 1977.

Abstract

Chloroplast DNA from Euglena gracilis was separated in an alkaline CsC1 density gradient into a heavy and a light component. The heavy and the light DNA components each contain 18 percent self-complementary nucleotide sequences. Equal masses of the heavy and the light components do not contain equal molar complementary nucleotide sequences. The light DNA component contains sequences complementary to the majority of the chloroplast DNA transcripts. The heavy DNA component contains sequences complementary to chloroplast ribosomal RNA.

Details

ISSN :
0006291X
Volume :
74
Database :
OpenAIRE
Journal :
Biochemical and Biophysical Research Communications
Accession number :
edsair.doi...........c817f5e4984ff7fd7e2a36bc68e8a349
Full Text :
https://doi.org/10.1016/0006-291x(77)91605-9