Study of the Bone-Biomaterial Interface Reactions in an in Vitro Bone Forming System: A Preliminary Report

An in vitro bone forming system is described in which interface reactions were studied with calcium phospates. A layer of hydroxyapatite or fluorapatite was applied on tissue culture plastic using the plasma-spray technique. Interactions between the bone forming cells and the extracellular matrix on one hand and the plasma-sprayed calcium phosphates on the other hand, were studied using light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray microanalysis (XRMA).