Additional file 1: of Modest heterologous protection after Plasmodium falciparum sporozoite immunization: a double-blind randomized controlled clinical trial

Authors :: Walk, Jona
Isaie Reuling
Behet, Marije
Meerstein-Kessel, Lisette
Graumans, Wouter
Geert-Jan Van Gemert
Siebelink-Stoter, Rianne
Vegte-Bolmer, Marga Van De
Janssen, Thorsten
Teelen, Karina
Wilt, Johannes De
Mast, Quirijn De
AndrĂŠ Van Der Ven
Benavente, Ernest Diez
Campino, Susana
Taane Clark
Huynen, Martijn
Cornelus Hermsen
Bijker, Else
Scholzen, Anja
Sauerwein, Robert
Publisher :: Figshare
Abstract: Protocol. Malaria antigen-specific IgG ELISA (S1). Isolation and cultivation of primary human hepatocytes (S2). In vitro sporozoite infectivity assay of primary human hepatocytes (S3). Immunofluorescent analysis of P. falciparum-infected primary human hepatocytes (S4). In vitro PBMC restimulation with PfRBCs and flow cytometry staining (S5) [11, 12, 14, 17, 42]. (DOCX 20 kb)

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