Back to Search Start Over

The murine Fc-gamma (Fcγ) receptor type II B1 is a tumorigenicity-enhancing factor in polyoma-virus-transformed 3T3 cells

Authors :
Catherine Sautes
Ilan Eliassi
Christian Bonnerot
Jos Even
Isaac P. Witz
Tal Zusman
Ofra Gohar
Yechiam Avivi
Ellen Lisansky
Wolf H. Fridman
Maya Ran
Source :
International Journal of Cancer. 65:221-229
Publication Year :
1996
Publisher :
Wiley, 1996.

Abstract

The murine receptor for the Fc portion of IgG is a molecule expressed by cells of the immune system. This study suggests the hypothesis that Fc gamma receptor type II B I (Fc gamma RIIB I) functions as a progression-enhancing factor when expressed ectopically on non-lymphoid tumor cells. It has been shown previously that BALB/c 3T3 cells transformed in vitro with polyoma virus (PyV) do not express Fc gamma RII but acquire the expression of this receptor following an in vivo passage in syngeneic mice. The specific Fc gamma RII transcript present in tumor cells was identified in this report as Fc gamma RIIB I (BI). In order to determine whether or not the ectopically expressed Fc gamma RII plays a role in the progression of these transformed cells, PyV-transformed 3T3 cells were transfected with BI-cDNA. The BI transfected cells were tested for their ability to form local tumors in syngeneic mice, as compared to transfected cells which express the co-transfecting neomycine resistance (neores) DNA alone or together with the lacZ gene. Fc gamma RIIB I expressors exhibited a significantly higher tumorigenic phenotype than FcR-negative controls, though both types of cells exhibited the same growth curve in vitro. The ability of Fc gamma RIIB I to act as a potentially tumorgenicity-enhancing factor was also demonstrated as Fc gamma RII was expressed by tumor cells, originating from inoculated Fc gamma RIIB I-transfected cells, or from inoculation of a mixture of receptor-positive and -negative cells. B I-expressing cells dominated the tumor-cell population over non-expressors. This dominance strengthened the hypothesis that FcR plays a role in tumor progression in vivo.

Details

ISSN :
10970215 and 00207136
Volume :
65
Database :
OpenAIRE
Journal :
International Journal of Cancer
Accession number :
edsair.doi...........c99a9b3015288866020092b492668e73
Full Text :
https://doi.org/10.1002/(sici)1097-0215(19960117)65:2<221::aid-ijc16>3.0.co;2-g