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Genetic Characterization and Detection of Angiostrongylus cantonensis by Molecular Approaches
- Source :
- Vector-Borne and Zoonotic Diseases. 21:643-652
- Publication Year :
- 2021
- Publisher :
- Mary Ann Liebert Inc, 2021.
-
Abstract
- Angiostrongylus cantonensis constitutes a major etiologic agent of eosinophilic meningoencephalitis. The detection methods for angiostrongyliasis mainly depend on morphology or immunology. A firmer diagnosis could be reached by directly detecting the parasite in the cerebrospinal fluid or through laboratory assays that are specific for Angiostrongylus-induced antibodies or the parasite's DNA. A. cantonensis detection could be carried out by larva release from the tissue upon pepsin digestion. However, the procedure requires live mollusks, which might complicate the analysis of large amounts of samples. Since morphological assays are limited, multiple molecular techniques have been put forward for detecting A. cantonensis, including PCR amplification of targets followed by fragment length or DNA sequence analysis. This allows rapid and accurate identification of A. cantonensis for efficient infection management and epidemiological purposes. In this study, we reviewed the current methods, concepts, and applications of molecular approaches to better understand the genetic characterization, molecular detection methods, and practical application of molecular detection in A. cantonensis.
- Subjects :
- Sequence analysis
Eosinophilic meningoencephalitis
Pepsin digestion
Computational biology
Biology
medicine.disease
biology.organism_classification
Microbiology
law.invention
Angiostrongylus cantonensis
Infectious Diseases
law
Virology
Angiostrongyliasis
medicine
Parasite hosting
Polymerase chain reaction
Subjects
Details
- ISSN :
- 15577759 and 15303667
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Vector-Borne and Zoonotic Diseases
- Accession number :
- edsair.doi...........cc02aa78a21c73a9e7313437bcca5286