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Enzymatic synthesis of myricetin 3-O-galactoside through a whole-cell biocatalyst
- Source :
- Chinese Herbal Medicines. 12:384-389
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Objective Myricetin 3-O-galactoside is an active compound with pharmaceutical potential. The insufficient supply of this compound becomes a bottleneck in the druggability study of myricetin 3-O-galactoside. Thus, it is necessary to develop a biosynthetic process for myricetin 3-O-galactoside through metabolic engineering. Methods Two genes OcSUS1 and OcUGE1 encoding sucrose synthase and UDP-glucose 4-epimerase were introduced into BL21(DE3) to reconstruct a UDP-D-galactose (UDP-Gal) biosynthetic pathway in Escherichia coli. The resultant chassis strain was able to produce UDP-Gal. Subsequently, a flavonol 3-O-galactosyltransferase DkFGT gene was transformed into the chassis strain producing UDP-Gal. An artificial pathway for myricetin 3-O-galactoside biosynthesis was thus constructed in E. coli. Results The obtained engineered strain was demonstrated to be capable of producing myricetin 3-O-galactoside, reaching 29.7 mg/L. Conclusion Biosynthesis of myricetin 3-O-galactoside through engineered E. coli could be achieved. This result lays the foundation for the large-scale preparation of myricetin 3-O-galactoside.
- Subjects :
- Pharmacology
Galactosyltransferase
biology
Chemistry
medicine.disease_cause
030226 pharmacology & pharmacy
01 natural sciences
Galactoside
0104 chemical sciences
Metabolic engineering
010404 medicinal & biomolecular chemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Complementary and alternative medicine
Biochemistry
Biosynthesis
Biosynthetic process
medicine
biology.protein
Sucrose synthase
Pharmacology (medical)
Myricetin
Escherichia coli
Subjects
Details
- ISSN :
- 16746384
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Chinese Herbal Medicines
- Accession number :
- edsair.doi...........cd409db3818591d0d104a811163e5060