Comparison of Two Assay Techniques to Study In Vitro Phagocytosis of Bovine Granulocytes Using Flow Cytometry

Authors :: Caudell, D.
McCourt, S.
Kalnitsky, J.
Ward, D.
Sriranganathan, N.
Saker, K. E.
Publication Year :: 2000
Publisher :: American Association of Bovine Practitioners Proceedings of the Annual Conference, 2000.
Abstract: The response of phagocytic cells to Pasteurella haemolytica is difficult to study in situ. Available assays to study the function of these cell types often do not accurately reflect the physiology of the cell in response to bovine pasteurellosis. This study compared granulocyte phagocytosis of fluorescent beads and fluorescent bacteria using flow cytometry. Development of an in vitro assay would provide a model to study nutrient and pharmaceutical effects on the immune response to Pasteurella infections. It would also allow for future studies of the pathogenesis of not only bovine pasteurellosis but other diseases.<br />American Association of Bovine Practitioners Proceedings of the Annual Conference, 2000

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