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Phosphorylation of Movement Proteins by the Plasmodesmal-Associated Protein Kinase
- Source :
- Plant Virology Protocols ISBN: 9781588298270
- Publication Year :
- 2008
- Publisher :
- Humana Press, 2008.
-
Abstract
- Plant viruses encode movement proteins (MPs) which play important roles in spreading their infectious materials throughout host plants. This infection is facilitated by cell-to-cell trafficking of MPs through specialized channels termed plasmodesmata, which involves specific interactions between MPs and host factors. Recently, we have reported the identification of a host protein kinase named plasmodesmal-associated protein kinase (PAPK) which specifically phosphorylates a subset of noncell autonomous proteins in vitro, including MPs of Tobacco mosaic virus (TMV) and Bean dwarf mosaic virus (BDMV). Biochemical purification of PAPK was achieved by developing a method in which a series of liquid chromatographic separations of plasmodesmal-enriched subcellular fractions was coupled with phosphorylation assays using TMV MP as a substrate. Application of this approach may prove useful in isolating other host kinases that interact with various viral components.
Details
- ISBN :
- 978-1-58829-827-0
- ISBNs :
- 9781588298270
- Database :
- OpenAIRE
- Journal :
- Plant Virology Protocols ISBN: 9781588298270
- Accession number :
- edsair.doi...........cfb9c6462825c4e7f02960435964b206