[Untitled]

Authors :: Antonello Pessi
Uwe Koch
Petra Neddermann
R. Bazzo
Elisabetta Bianchi
Sonia Sambucini
Paolo Ingallinella
Gaetano Barbato
De Francesco R
Daniel O. Cicero
Source :: Journal of Biomolecular NMR. 20:23-29
Publication Year :: 2001
Publisher :: Springer Science and Business Media LLC, 2001.
Abstract: A new isotope-filtered experiment has been designed to measure homonuclear three-bond J(H(N)Halpha) coupling constants of unlabeled peptides complexed with labeled proteins. The new experiment is based on the 3D HNHA pulse scheme, and belongs to the 'quantitative J-correlation' type. It has been applied to a decapeptide inhibitor bound to the proteinase domain of the NS3 protein of human hepatitis C virus (HCV).