Quantification of circulating microRNA using TaqMan Low Density Array (TLDA) v1

A protocol for quantification of circulating microRNA using TaqMan Low Density Array. MicroRNAs are purified from platelet-poor plasma using Nucleospin columns During the purification step samples are spiked with cel-miR-39 as a mean of technical normalization Normalization is performed using the ΔCt-method