Back to Search
Start Over
Hydrazone derivative bearing coumarin for the relay detection of Cu2+ and H2S in an almost neat aqueous solution and bioimaging in lysosomes
- Source :
- Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 255:119693
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- A new morpholine functionalized coumarin-based fluorescent probe 1 was easily synthesized. The probe realized the sequentially detecting of Cu2+ and H2S in the HEPES buffer solution (20 mM, pH = 5.0). It made a turn-off fluorescence response to Cu2+ by using a complex formation with a 2:1 binding mode, and the resulting complex was able to detect H2S according to the displacement approach with a turn-on fluorescence response. The detecting limits of probe 1 for Cu2+ and 1–Cu2+ system for H2S were calculated to be 26 nM and 88.5 nM, respectively. This “on-off-on” recognition process was demonstrated by ultraviolet–visible spectroscopy, fluorescence spectroscopy, using proton nuclear magnetic resonance studies, electrospray ionization mass spectroscopy, single crystal X-ray diffraction, and using density functional theory calculations. In addition, both cell imaging and co-staining experiments showed that the probe could be utilized to visually detect Cu2+ and H2S in lysosomes.
- Subjects :
- Aqueous solution
Chemistry
Electrospray ionization
02 engineering and technology
010402 general chemistry
021001 nanoscience & nanotechnology
Mass spectrometry
Photochemistry
01 natural sciences
Fluorescence
Atomic and Molecular Physics, and Optics
Fluorescence spectroscopy
0104 chemical sciences
Analytical Chemistry
chemistry.chemical_compound
Morpholine
Proton NMR
0210 nano-technology
Spectroscopy
Instrumentation
Subjects
Details
- ISSN :
- 13861425
- Volume :
- 255
- Database :
- OpenAIRE
- Journal :
- Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
- Accession number :
- edsair.doi...........d138bc03d03e9a1cc36aeda2778d444c