1123 ESTABLISHMENT OF PRIMARY TUMOR XENOGRAFTS IN IMMUNODEFICIENT MICE - THE ROLE OF CHRONIC GRAFT VERSUS HOST REACTIONS - IDENTIFICATION AND AVOIDANCE OF INVALID SCIENTIFIC RESULTS

and ChIP test whether AIB1 is necessary for glycolytic genes transcription. The independent statistics analysis of microarray was employed to identify the correlation between AIB1 and glycolytic genes. RESULTS: Our results demonstrated knockdown of AIB1 in bladder cancer cells induced G1 arrest and Our results demonstrated knockdown of AIB1 in bladder cancer cells induced G1 arrest and apoptosis, inhibited colony formation and tumorigenesis in vivo. Cell cycle inhibitor, p27, was up-regulated and survival protein BCL2 was reduced in AIB1 knockdown cells independence of cell type. Depletion of AIB1 by RNA interference and pharmacologic inhibition reduced glucose consumption, lactate and ATP production in T24 cancer cells under hypoxia. By interacting with HIF1 , AIB1 was required for HIF1 transcriptional activity and its glycolytic target gene expression, through direct recruitment onto promoters of HIF1 target genes. AIB1expression level showed positive correlation with glycolytic genes in clinic UCB specimen. CONCLUSIONS: Together, oveexpression of AIB1 is required for glycolysis in bladder cancer cells through HIF1 , eventually to facilitate tumorigenesis. Targeting AIB1 by gossypol may be an intriguing approach for bladder cancer therapy.