Biometric and Genetic Stability Assessment of In Vitro Rooted Populus alba L. Micro-Shoots

Populus alba is a deciduous large woody tree. The plant was introduced to shooting, then rooting multiplication on MS media. This work's design depends on two factors (media power and sucrose concentrations) with different concentrations resulted in 12 treatments. There were nine responses measured in the new individuals. Some morphological parameters had a significant difference, such as shoot length, leaf and root numbers. Where in the physiological measurements, all the parameters measured had significant differences. RAPD-PCR molecular marker was applied to assess the variation in the new individuals' genetic stability from treatments with the two factors. Four RAPD primers gave reproducible bands and resulted in a total polymorphism percentage of 38.33%. The new individuals resulted from the tissue culture technique were identical to the mother plant and each other. However, in this work, the use of different media power and sucrose concentrations resulted in different values in morphological, physiological responses, and P. alba's molecular variation.