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Cloning and characterization of glucosyltransferase cDNA from Eucalyptus perriniana cultured cells
- Source :
- Plant Biotechnology. 21:343-348
- Publication Year :
- 2004
- Publisher :
- Japanese Society for Plant Cell and Molecular Biology, 2004.
-
Abstract
- The Eucalyptus perriniana cultured cells are widely used to biotransform a variety of compounds. The glucosyltransferase activity of a crude protein extract of E. perriniana cultured cells was maximized when cell growth was in the pre-logarithmic to logarithmic phase. We cloned a cDNA encoding glucosyltransferase (EPGT) from E. perriniana cultured cells by RT-PCR using a degenerated primer and RACE-PCR. The cDNA contained an open reading frame encoding 467 amino acids with a calculated molecular mass of 51.6 kDa. The consensus sequence of the plant glucosyltransferases was included in the deduced amino acid sequence. The amino acid sequence of EPGT showed a high identity to glucosyltransferases from tobacco and petunia. The recombinant EPGT was expressed in Escherichia coli and its substrate specificity was examined using UDP-(U- 14 C) glucose. Cinnamic acid was the best sugar acceptor in the compounds tested.
- Subjects :
- chemistry.chemical_classification
biology
Plant Science
biology.organism_classification
medicine.disease_cause
Molecular biology
Eucalyptus perriniana
Amino acid
Glucosyltransferases
chemistry
Biochemistry
Complementary DNA
biology.protein
Consensus sequence
medicine
Glucosyltransferase
Agronomy and Crop Science
Escherichia coli
Peptide sequence
Biotechnology
Subjects
Details
- ISSN :
- 13476114 and 13424580
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Plant Biotechnology
- Accession number :
- edsair.doi...........d27e04ae4e32bb6141d9d08eba1be790