Preventive Effect and Mechanism of Cannabis sativa Ingredient on Acute Coronary Artery by Inhibition of Endothelial Cell Inflammatory Response

The Chinese medicine of Cannabis sativa fruit is called “Hemp Seed” or “Cannabis Kernel.” It is calm, sweet, and has the function of moistening the intestines and treating dry stool. The flower is called “Ma Bo,” and it treats evil wind, amenorrhea, and forgetfulness. After the extraction of active ingredient from Cannabis sativa, its treatment activity against acute coronary artery was determined and the detailed mechanism was also examined. First of all, ELISA was exploited to investigate the release of inflammatory cytokines in plasma. After that, via utilizing real time RT-PCR, the activation for the signaling pathway of NF-κB existing in endothelial cell could be identified after the specific treatment. For the Cannabis sativa, its complete chloroplast genome was analyzed through Illumina pair-end sequencing. According to neighbor-joining (NJ) phylogenetic analysis, the Humulus lupulus and Cannabis sativa come together and become the sisters of other associated species.