Optimization of a Pravastatin Quantification Method Using HPLC with Ultraviolet Detection in Human Serum for Monitoring Dyslipidemic Patients

A high performance liquid chromatography (HPLC) method for the estimation of pravastatin in human serum samples has been developed to monitor dyslipidemic patients. The method was fully validated and validation parameters were in the linearity range 10–200 ng/mL, correlation coefficient 0.99, mean recovery >0.73, quantification limit 10 ng/mL, and limit of detection, 5 ng/mL; this method was applied for pravastatin determination in human serum from Mexican dyslipidemic patients. Pravastatin values found for three studied patients were 73, 57, and