Supplementation of laying-hen feed with annatto tocotrienols and impact of α-tocopherol on tocotrienol transfer to egg yolk and tocopherol and annatto tocotrienol distribution analysis in laying-hen body

vi CHAPTER 1: GENERAL INTRODUCTION 1 Egg Nutrition 1 Rational for Nutrient Enrichment 2 Rational for Distribution Determination 5 Thesis Organization 5 References 6 CHAPTER 2: LITERATURE REVIEW 9 Vitamin E: Tocopherols and Tocotrienols 9 Absorption and Transport Vitamin E 12 Annatto Tocotrienols 14 Cholesterol Lowering 15 Cholesterol Quantification 18 Egg Formation 19 References 22 CHAPTER 3: SUPPLEMENTATION OF LAYING-HEN FEED WITH ANNATTO TOCOTRIENOLS AND IMPACT OF α-TOCOPHEROL ON TOCOTRIENOL TRANSFER TO EGG YOLK 27 Abstract 28 Introduction 29 Materials and Methods 30 Results and Discussion 36 Conclusions 46 References 48 Tables and Figures 5428 Introduction 29 Materials and Methods 30 Results and Discussion 36 Conclusions 46 References 48 Tables and Figures 54