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Delivering the cell-impermeable DNA ‘light-switching’ Ru(<scp>ii</scp>) complexes preferentially into live-cell nucleus via an unprecedented ion-pairing method
- Source :
- Chemical Science. 7:4016-4023
- Publication Year :
- 2016
- Publisher :
- Royal Society of Chemistry (RSC), 2016.
-
Abstract
- The dipyridophenazine (dppz) based ruthenium polypyridyl complexes are known as molecular ‘light-switches’ for DNA. This property is poised to serve in diagnostic and therapeutic applications, but the poor cellular uptake restricts their use in live cells. Herein, we show that the cellular uptake, and more interestingly and surprisingly, the nuclear uptake of cell-impermeable Ru(II)–polypyridyl cationic complexes such as [Ru(bpy)2(dppz)]2+ were remarkably enhanced by three structurally unrelated biochemical agents (pentachlorophenol, carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone and tolfenamic acid), by forming lipophilic and relatively stable ion-pair complexes, via a passive diffusion mechanism. Enantioselective imaging of live-cell nuclear DNA was observed between the two chiral forms of Ru(II) complexes. This represents the first report of an unprecedented new method for delivering the DNA ‘light-switching’ Ru(II) complexes into the nucleus of living cells via ion-pairing, which could serve as a promising general live-cell delivery method for other potentially bio-medically important but cell-impermeable metal complexes.
- Subjects :
- 010405 organic chemistry
Enantioselective synthesis
Cationic polymerization
chemistry.chemical_element
General Chemistry
010402 general chemistry
01 natural sciences
Combinatorial chemistry
0104 chemical sciences
Ruthenium
Nuclear DNA
Metal
chemistry.chemical_compound
medicine.anatomical_structure
Tolfenamic acid
chemistry
visual_art
visual_art.visual_art_medium
medicine
Organic chemistry
Nucleus
DNA
medicine.drug
Subjects
Details
- ISSN :
- 20416539 and 20416520
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Chemical Science
- Accession number :
- edsair.doi...........d7f670166f6f8632a3b5376c0ae4dbd2