Airways mucus pathogenesis in patients with non-cystic fibrosis bronchiectasis

Background: Non-cystic fibrosis bronchiectasis (NCFB) is a chronic airway condition characterized by the production of purulent sputum, prolonged inflammation, and repeated episodes of airway infection. We assessed the biochemical and biophysical properties of airway mucous in patients with NCB and healthy adults. Methods: Induced sputum was collected in 99 patients with NCFB and 15 healthy adults. We assessed the sputum samples for mucus percent solids (% solids), total mucin (refractometry), extracellular DNA (ELISA), osmotic pressure (custom-made oncometer), complex viscosity (cone and plate rheology), and sputum proteomics (mass spectrometry). Data are presented as mean (standard deviation). Results: Patients with NCFB were older (62.3 years (10.3)) and had lower FEV1 (66.3% (18.1)) compared with controls (42.2 years (19.3); 99.9% (9.0)). Sputum from patients with NCFB had higher mucus percent solids (3.3% (1.9) vs. 1.2% (0.5)), mucin concentration (5, 612µg/ml (3, 327) vs. 1, 637µg/ml (1030)), and DNA concentration (435µg/ml (487) vs. 22µg/ml (26)) compared with controls (p Conclusion: Airway mucous in patients with NCFB is biochemically and biophysically abnormal compared with healthy airways. Mucus hyper-concentration likely contributes to infection, inflammation, and impaired mucociliary clearance in NCFB lung disease.