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Intrahippocampal 5-HT1A receptor antagonist inhibits the improving effect of low-frequency stimulation on memory impairment in kindled rats
- Source :
- Brain Research Bulletin. 148:109-117
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- In addition to its anticonvulsant effect, low frequency stimulation (LFS) improves learning and memory in kindled animals. In the present study, the role of 5-HT 1A receptors in mediating LFS’ improving effect on spatial learning and memory was investigated in amygdala-kindled rats. Amygdala kindling was conducted in a semi-rapid kindling stimulations (12 stimulations per day) in male Wistar rats. LFS (4 trains of 0.1 ms pulse duration at 1 Hz, 200 pulses, 50–150 μA, at 5 min intervals) was applied after termination of kindling stimulations. NAD-299 (a selective 5-HT 1A receptor antagonist; 2.5 and 5 μg/μl) was microinjected into the hippocampal CA1 before applying LFS. The Morris water maze, and novel object recognition tests were conducted after the last kindling stimulation. Hippocampal samples were also prepared, and 5-HT 1A receptor gene expression levels were assessed using quantitative RT-PCR. In kindled animals, LFS reduced impairments in spatial learning and memory in the Morris water maze and novel object recognition tests. Microinjection of NAD doses of 5 μg/μl reduced the effects of LFS on learning and memory. The gene expression level of 5-HT 1A receptors increased significantly in the hippocampus of amygdala-kindled rats. However, LFS applied after kindling stimulations inhibited this effect. It seems that activation of 5-HT 1A receptors in the CA1 field is necessary for LFS’ improving effects on spatial learning and memory in kindled animals; although surprisingly, LFS application prevented the elevation in gene expression of 5-HT 1A receptors in kindled animals. © 2019 Elsevier Inc.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
medicine.drug_class
Chemistry
Kindling
General Neuroscience
Antagonist
Morris water navigation task
Hippocampus
Stimulation
Hippocampal formation
Receptor antagonist
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Endocrinology
Internal medicine
medicine
5-HT1A receptor
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 03619230
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Brain Research Bulletin
- Accession number :
- edsair.doi...........d97b7f14cd167dce8ec495d554bee45f