Effects of Voltage and Distance on Stand-Alone Lance Electrodes During Repulsion

This paper explores the effects of two parameters—operation voltage and the distance between the lance and electrode—on the repulsion process of a Stand-Alone Lance, a second-generation Nanoinjection tool used to inject DNA into egg cells for genetic purposes. Using a mathematical computer model to simulate the motion of the DNA during repulsion from the lance and into the cell, the results are quantified by the average DNA travel time and the electric field strength, which relates to the electroporation envelope. It is found that voltage has a very strong effect, while changing distance yields much less dramatic results. Specific ideal values are recommended for the magnitude and duration of the voltage pulse to successfully repel DNA into the cell.Copyright © 2012 by ASME