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Tetrahedral Framework Nucleic Acids Loading Ampicillin Improve the Drug Susceptibility against Methicillin-Resistant Staphylococcus aureus

Authors :
Sirong Shi
Yunfeng Lin
Qirong Li
Yuxin Zhang
Songhang Li
Lingxian Meng
Dan Zhao
Yue Sun
Taoran Tian
Xueping Xie
Source :
ACS Applied Materials & Interfaces. 12:36957-36966
Publication Year :
2020
Publisher :
American Chemical Society (ACS), 2020.

Abstract

The overuse of antibiotics has led to the emergence of multidrug-resistant pathogens. There is an urgent need to develop alternative therapeutic strategies to reduce mortality and morbidity related to drug-resistant bacterial infections. Self-synthesized tetrahedral framework nucleic acids (tFNAs) are used as the drug loading platform to deliver ampicillin to combat methicillin-resistant Staphylococcus aureus (MRSA) infection. The results of average dimension, zeta potential, transmission electron microscopy, and ultraviolet spectrophotometry showed that tFNAs-ampicillin combined with a sufficient encapsulation rate and good stability. tFNAs-ampicillin had a better affinity to MRSA than free ampicillin because it had a better uptake by MRSA cells. Additionally, tFNAs-ampicillin had a better antibacterial effect and lower levels of resistance development than free ampicillin. The downregulation of genes related to bacterial cell wall synthesis (murA and murZ) and upregulation of a gene related to antibiotic sensibility (PBP2) were responsible for the enhanced killing effect of tFNAs-ampicillin against MRSA.

Details

ISSN :
19448252 and 19448244
Volume :
12
Database :
OpenAIRE
Journal :
ACS Applied Materials & Interfaces
Accession number :
edsair.doi...........dcbaa6d261adf6c920545e9a74fcdfdb
Full Text :
https://doi.org/10.1021/acsami.0c11249