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Adaptive Laboratory Evolution for algal strain improvement: methodologies and applications

Adaptive Laboratory Evolution for algal strain improvement: methodologies and applications

Authors :
Alaina J. LaPanse
Matthew C. Posewitz
Anagha Krishnan
Source :
Algal Research. 53:102122
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

Originally developed in bacterial and fungal models, Adaptive Laboratory Evolution (ALE) experimentally selects for targeted phenotypes by allowing cells containing beneficial random genetic mutations to replicate more quickly in the presence of environmental stress. When applied to microalgae, this technique has been used to increase growth rate, stress tolerance, substrate utilization, and product yield. This review discusses ALE experimental design parameters, and summarizes key results and lessons learned from recent microalgal ALE studies. By documenting the current state of the field, we provide insight on the ways microalgal ALE might be used to develop commercially viable biotechnology strains.

Details

ISSN :
22119264
Volume :
53
Database :
OpenAIRE
Journal :
Algal Research
Accession number :
edsair.doi...........dd6887e589c7a7b7b37119d2b0e65c75
Full Text :
https://doi.org/10.1016/j.algal.2020.102122