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Development of 26 SNP markers in Dabry’s sturgeon (Acipenser dabryanus) based on high-throughput sequencing

Authors :
Huifang Jing
Xiaodong Wang
Jiuxuan Li
Qingzhan Ma
Kan Liu
Zhaobin Song
Source :
Conservation Genetics Resources. 9:205-207
Publication Year :
2016
Publisher :
Springer Science and Business Media LLC, 2016.

Abstract

Dabry’s sturgeon (Acipenser dabryanus) is an endemic freshwater species in China mainly distributed in the mainstream of the upper Yangtze River and its tributaries. Due to river fragmentation, sand/gravel extraction, over-fishing, and water pollution, the wild population of Dabry’s sturgeon suffered a dramatic decline. In the present study, 26 novel single nucleotide polymorphism (SNP) markers were developed based on high-throughput sequencing. Genetic diversity analysis revealed that the values of observed heterozygosity (Ho) and expected heterozygosity (He) varied from 0.1000 to 0.9000 and from 0.0974 to 0.5115, respectively, and the minor allele frequency ranged from 0.0500 to 0.4750. Only three loci showed significant deviation from Hardy–Weinberg equilibrium after Bonferroni correction. The SNP markers will serve as a valuable tool for further population genetics, genetic breeding, and resource conservation in this species.

Details

ISSN :
18777260 and 18777252
Volume :
9
Database :
OpenAIRE
Journal :
Conservation Genetics Resources
Accession number :
edsair.doi...........de738d7e81ee336ba3dd1f5ea41e116b
Full Text :
https://doi.org/10.1007/s12686-016-0651-7