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Fluorescence calibration research of microspheres in a microfluidic chip

Authors :
Kun Lian
Jost Goettert
Lin Wang
Source :
SPIE Proceedings.
Publication Year :
2005
Publisher :
SPIE, 2005.

Abstract

Fluorescence calibration is usually done by preparing bio-samples with a series of concentrations and measuring their corresponding fluorescence intensities. A simplified approach is studied by using a microfluidic chip and microspheres. The fluorescence calibration can be carried out on the chip with only one concentration of the microspheres. Microspheres with the diameter of 1~5μm are very useful in bio-detection research. These microspheres are manufactured using high-quality, ultraclean polystyrene microspheres and loaded with a variety of proprietary dyes. They can be labeled with biotin-, NeutrAvdin-, streptavidin- and protein, which can be used as tracers for bio-detections. A microfluidic chip was successfully fabricated for the experiment, and preliminary experimental results have proved the feasibility of the approach for fluorescence calibration.

Details

ISSN :
0277786X
Database :
OpenAIRE
Journal :
SPIE Proceedings
Accession number :
edsair.doi...........dea4a39ad80bb0f7be2b71466f6e9698
Full Text :
https://doi.org/10.1117/12.591610