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The Extract from Acidosasa longiligula Alleviates in vitro UV-Induced Skin Cell Damage via Positive Regulation of Thioredoxin 1
- Source :
- Clinical Interventions in Aging. 15:897-905
- Publication Year :
- 2020
- Publisher :
- Informa UK Limited, 2020.
-
Abstract
- Introduction Skin, as the outermost organ, is exposed to a wide range of environmental risk factors including ultraviolet (UV) and all kinds of pollutants. Excessive UV exposure contributes to many disorders, such as photoaging, skin inflammation, and carcinogenesis. Methods To determine the effects of bamboo extract (BEX) from our local plant, Acidosasa longiligula, on UV-irritated human skin, we conducted a variety of studies, including Western blot, apoptosis assays, reactive oxygen species (ROS) detection, and thioredoxin (TXN) and thioredoxin reductase (TXNRD) activity assays in primary skin keratinocytes. Results We first determined that BEX protects human skin keratinocytes against UV radiation-induced apoptosis and ROS production. UV radiation can robustly impair TXN and TXNRD activity which can, in turn, be significantly rescued by BEX treatment. Moreover, BEX regulates TXN1 levels in primary skin keratinocytes and TXN1 is proved to be required for the protective function of BEX. Last, we found that the NF-κB/p65 pathway mediates the protective function of BEX against UV. Discussion Collectively, our work delineates the beneficial role of BEX in UV-induced skin cell damage and provides a novel therapeutic reagent to prevent or alleviate the progress of photoaging and other UV-provoked skin diseases.
- Subjects :
- chemistry.chemical_classification
Reactive oxygen species
integumentary system
medicine.diagnostic_test
business.industry
Thioredoxin reductase
Photoaging
Human skin
General Medicine
medicine.disease
medicine.disease_cause
Cell biology
03 medical and health sciences
0302 clinical medicine
chemistry
Western blot
Apoptosis
medicine
030212 general & internal medicine
Geriatrics and Gerontology
Thioredoxin
business
Carcinogenesis
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 11781998
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Clinical Interventions in Aging
- Accession number :
- edsair.doi...........df3cb30c7569ba55071c19556921905a