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Antiestrogenic Activity of Extracts of Diesel Exhaust Particulate Matter in MCF-7 Human Breast Carcinoma Cells
- Source :
- Polycyclic Aromatic Compounds. 22:747-759
- Publication Year :
- 2002
- Publisher :
- Informa UK Limited, 2002.
-
Abstract
- Diesel exhaust particulate matter (DEP) was collected from three commercial diesel engine vehicles and DEP extracts (DEPEs) were prepared from each. The estrogenic and antiestrogenic activities of the DEPE samples were examined using an estrogen response element (ERE)-driven luciferase reporter gene assay in MCF-7 human breast cancer cells. While DEPE samples did not exhibit significant estrogen agonist activity alone, they suppressed 17 g -estradiol (E 2 )-induced luciferase activity without decreasing cell viability. Several polycyclic aromatic hydrocarbons (PAHs) acting as aryl hydrocarbon receptor (AhR) agonists were detected in the DEPEs and their concentrations were higher in DEPE samples with higher antiestrogenic activity. DEPE samples elevated cytochrome P450 (CYP) 1A1 activity. f -Naphthoflavone, an AhR antagonist, and SKF-525A, a nonselective CYP inhibitor, reversed and enhanced the antiestrogenic activity of DEPE samples, respectively. These results demonstrate that DEPE has antiestrogenic act...
- Subjects :
- chemistry.chemical_classification
Polymers and Plastics
biology
Chemistry
Organic Chemistry
Estrogen receptor
Polycyclic aromatic hydrocarbon
Cytochrome P450
respiratory system
Aryl hydrocarbon receptor
Molecular biology
Diesel Exhaust Particulate
MCF-7
Materials Chemistry
biology.protein
Organic chemistry
Luciferase
Viability assay
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 15635333 and 10406638
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Polycyclic Aromatic Compounds
- Accession number :
- edsair.doi...........e311446ea66025ee8258e7fb9fcef2ab