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Microfluidic platform versus conventional real-time polymerase chain reaction for the detection of Mycoplasma pneumoniae in respiratory specimens
- Source :
- Diagnostic Microbiology and Infectious Disease. 67:22-29
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine or outpatient implementation. Here, we evaluate a novel microfluidic real-time PCR platform (Advanced Liquid Logic, Research Triangle Park, NC) that is rapid, portable, and fully automated. We enrolled patients with CAP and extracted DNA from nasopharyngeal wash (NPW) specimens using a biotinylated capture probe and streptavidin-coupled magnetic beads. Each extract was tested for M. pneumoniae-specific DNA by real-time PCR on both conventional and microfluidic platforms using Taqman probe and primers. Three of 59 (5.0%) NPWs were positive, and agreement between the methods was 98%. The microfluidic platform was equally sensitive but 3 times faster and offers an inexpensive and convenient diagnostic test for microbial DNA.
- Subjects :
- Microbiology (medical)
Mycoplasma pneumoniae
Microbial DNA
Microfluidics
General Medicine
Biology
medicine.disease_cause
Molecular biology
Microbiology
law.invention
chemistry.chemical_compound
Infectious Diseases
Real-time polymerase chain reaction
chemistry
law
Biotinylation
medicine
TaqMan
DNA
Polymerase chain reaction
Subjects
Details
- ISSN :
- 07328893
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- Diagnostic Microbiology and Infectious Disease
- Accession number :
- edsair.doi...........e5895ee90920f8df8ed66eeb9f116d1c