In-vivo corneal biomechanics measurement by 3D trackable Brillouin microscopy

Brillouin microscopy has been applied successfully to in-vivo corneal imaging to understand stiffness variation along different phases of keratoconus. However, the in-vivo spatial resolution is restricted due to a conflict between a slow Brillouin acquisition rate of hundreds of milliseconds and human movement during acquisition. In this research, frequency domain optical coherence tomography and image tracking are proved to be effective methods to locate points measured by Brillouin microscopy in axial and radial directions, respectively. Results show that OCT has an accuracy of better than 2 μm while image tracking has an accuracy better than 3 μm.