Back to Search
Start Over
Optimum Preparation Method for Self-Assembled PEGylation Nano-Adjuvant Based on Rehmannia glutinosa Polysaccharide and Its Immunological Effect on Macrophages
- Source :
- International Journal of Nanomedicine. 14:9361-9375
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- Background Rehmannia glutinosa polysaccharide is the main reason that contributes to the immunological function of R. glutinosa. Due to its disadvantages in clinical use, here we designed the PEGylation nano-RGP (pRL) to improve the drug-targeting effect and the immunological function. Our present work aims to establish the optimum condition of preparing the pRL and to investigate its immunological function on macrophages. Methods pRL was prepared by thin film hydration method combined with ultra-sonication technique. And its preparation conditions were optimized with response surface methodology. Also, the lyophilization method was optimized. The characteristics of the pRL were evaluated, including particle size, drug loading, encapsulation efficiency and morphology. The immunological function of pRL on macrophage was investigated through CCK-8 test, ELISA and flow cytometry. Results The lipid-to-cholesterol molar ratio of 8:1, the addition of DSPE-PEG2000 of 9% and the lipid-to-drug ratio of 5.4:1 were the optimum preparation technology for pRL. The encapsulation efficiency (EE) of pRL under this preparation technology was 95.81±1.58%, with a particle size of 31.98 ± 2.6 nm. The lactose-to-lipid ratio (2:1) was the optimal lyophilization method. pRL promoted macrophage proliferation, which is significantly better than that of nano-RGP without PEGylation (RL). pRL-stimulated RAW264.7 cells showed a high secretion of pro-inflammatory cytokines, which is the characteristic indicator of M1 polarization. Enhanced cellular uptake through macropinocytosis-dependent and caveolae-mediated endocytosis was observed in pRL-treated RAW264.7 cells. Conclusion Our study concluded that PEGylation effectively overcame the poor targeting effect of Rehmannia glutinosa polysaccharide (RGP) and significantly improved the immunological profile of its nano-formulation, which suggested that pRL could serve as an immune adjuvant in clinical application.
- Subjects :
- endocrine system
medicine.medical_treatment
Biophysics
Pharmaceutical Science
Bioengineering
02 engineering and technology
Immunopotentiator
010402 general chemistry
Polysaccharide
Endocytosis
01 natural sciences
Flow cytometry
Biomaterials
Drug Discovery
medicine
chemistry.chemical_classification
biology
medicine.diagnostic_test
Organic Chemistry
General Medicine
021001 nanoscience & nanotechnology
Rehmannia glutinosa
biology.organism_classification
0104 chemical sciences
Biochemistry
chemistry
PEGylation
0210 nano-technology
Adjuvant
Macrophage proliferation
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 11782013
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi...........e789adc3590b4faea2bcad5a62402904